Pyr pUAST-HA
(Plasmid #69768)

• Purpose: Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
• Depositing Lab: Arno Muller
• Publication: Klingseisen et al Development. 2009 Jul;136(14):2393-402. doi: 10.1242/dev.035451. Epub 2009 Jun 10.

Backbone

• Vector backbone: pUAST-HA
• Backbone manufacturer: ?
• Backbone size w/o insert (bp): 8900
• Total vector size (bp): 11201
• Vector type: Insect Expression ; P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pyramus (FGF8like-2)
• Alt name: Pry, FGF8like-2 ligand
• Species: D. melanogaster (fly)
• Insert Size (bp): 2301
• Mutation: 241T amino acid insertion compared to reference sequence AAS90558.2
• Entrez Gene: pyr (a.k.a. Dmel_CG13194, CG13194, DFGF8-2, DmPyr, Dmel\CG13194, FGF8-like2, Pyr, fgf8-like)
• Promoter: hsp70 promoter
• Tag / Fusion Protein:
  • HA-Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: pUAST for

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Anna Klingseisen, Alumni

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry