pUAST-HA, Pbl-A
(Plasmid #69792)

• Purpose: Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
• Depositing Lab: Arno Muller
• Publication: van Impel et al Development. 2009 Mar;136(5):813-22. doi: 10.1242/dev.026203. Epub 2009 Feb 11.

Backbone

• Vector backbone: pUAST-HA
• Backbone manufacturer: ?
• Backbone size w/o insert (bp): 8900
• Total vector size (bp): 11450
• Modifications to backbone: HA tag
• Vector type: Insect Expression ; P element-based pUAST vector for Gal4-regulated expression in Drosophila.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB media
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pebble - Drosophila guanine nucleotide exchange factor
• Alt name: Pbl, Pbl-2A
• Species: D. melanogaster (fly)
• Insert Size (bp): 2559
• Mutation: Encodes Pbl (NP_729306.1) lacking last 9 amino acids.
• Promoter: hsp70 promoter
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: pUAST for

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Sabine Schumacher, Alumni

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry