pCMV-SPORT6-RNF114-UIMmut
(Plasmid
#69923)
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PurposeExpresses mutant myc-RNF114 in mammalian cells
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Depositing Lab
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Depositing OrganizationKing's College London
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69923-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69923-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV-SPORT6
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4400
- Total vector size (bp) 5100
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRNF114
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Alt nameZNF313
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SpeciesH. sapiens (human)
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Insert Size (bp)700
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MutationS224P
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Entrez GeneRNF114 (a.k.a. PSORS12, ZNF313)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69923-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69923-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-SPORT6-RNF114-UIMmut was a gift from Francesca Capon (Addgene plasmid # 69923 ; http://n2t.net/addgene:69923 ; RRID:Addgene_69923) -
For your References section:
Functional analysis of the RNF114 psoriasis susceptibility gene implicates innate immune responses to double-stranded RNA in disease pathogenesis. Bijlmakers MJ, Kanneganti SK, Barker JN, Trembath RC, Capon F. Hum Mol Genet. 2011 Aug 15;20(16):3129-37. doi: 10.1093/hmg/ddr215. Epub 2011 May 13. 10.1093/hmg/ddr215 PubMed 21571784