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Purpose(Empty Backbone) bacterial expression of MBP-TEV n-terminal fusions
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Depositing Labs
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Depositing OrganizationAston University
Located in United Kingdom of Great Britain and Northern Ireland -
Publication
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Citations
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET28
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Backbone manufacturerNovagen
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Modifications to backboneWe cloned the ORF fragment encoding K27 to K396 of the E. coli malE protein together with a TEV protease cleavage site (ENLYFQGS) using the NheI and BamHI restriction sites of the pET28 bacterial expression plasmid (Novagen)
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Vector typeBacterial Expression
- Promoter T7-lacO (lactose/IPTG inducible)
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Tags
/ Fusion Proteins
- MBP (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-MBP-TEV was a gift from Zita Balklava & Thomas Wassmer (Addgene plasmid # 69929 ; http://n2t.net/addgene:69929 ; RRID:Addgene_69929) -
For your References section:
APP controls the formation of PI(3,5)P vesicles through its binding of the PIKfyve complex. Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T. Cell Mol Life Sci. 2015 Jul 28. 10.1007/s00018-015-1993-0 PubMed 26216398
