|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69929||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||69929-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Modifications to backboneWe cloned the ORF fragment encoding K27 to K396 of the E. coli malE protein together with a TEV protease cleavage site (ENLYFQGS) using the NheI and BamHI restriction sites of the pET28 bacterial expression plasmid (Novagen)
Vector typeBacterial Expression
- Promoter T7
/ Fusion Protein
- MBP-TEV (N terminal on backbone)
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 69929-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-MBP-TEV was a gift from Zita Balklava & Thomas Wassmer (Addgene plasmid # 69929 ; http://n2t.net/addgene:69929 ; RRID:Addgene_69929)
For your References section:APP controls the formation of PI(3,5)P vesicles through its binding of the PIKfyve complex. Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T. Cell Mol Life Sci. 2015 Jul 28. 10.1007/s00018-015-1993-0 PubMed 26216398