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pLVE-FH-IN
(Plasmid #70071)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70071 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-tTRKRAB-red
  • Backbone manufacturer
    Dr. Didier Trono, Addgene #12250
  • Modifications to backbone
    added FLAG-HA-IRES-neoR
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Promoter EF1alpha
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The DNA sequences of the IRES and neomycin resistant gene (neoR) were PCR amplified from pLV-tRKRAB-red (Dr. Didier Trono, Addgene plasmid 12250) and pEGFP-N1 (Clontech), respectively. 3' UTR of mouse Eef1a1 (NM_010106.2) was obtained from mouse embryonic stem cells by RT-PCR. Then, a BamHI-IRES-neoR (IN)-EcoRI-3'UTR of Eef1a1 (F)-Kpn1 fragment was synthesized by PCR flanked with PspXI-FLAG-HA(FH)-BamHI at the 5' end. Then the PspXI-FH-BamHI-IRES-neoR-EcoRI-E-KpnI fragment was subcloned into PspXI and KpnI sites of pLV-tTRKRAB-red to create pLVE-FH-IN.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVE-FH-IN was a gift from Thomas Tuschl (Addgene plasmid # 70071 ; http://n2t.net/addgene:70071 ; RRID:Addgene_70071)
  • For your References section:

    DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T. Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. 10.1038/nature21690 PubMed 28297718