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hSERT-N1-pEYFP
(Plasmid #70105)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70105 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SERT
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSERT-N1-pEYFP was a gift from Harald Sitte (Addgene plasmid # 70105 ; http://n2t.net/addgene:70105 ; RRID:Addgene_70105)
  • For your References section:

    Identification of an additional interaction domain in transmembrane domains 11 and 12 that supports oligomer formation in the human serotonin transporter. Just H, Sitte HH, Schmid JA, Freissmuth M, Kudlacek O. J Biol Chem. 2004 Feb 20;279(8):6650-7. Epub 2003 Dec 5. 10.1074/jbc.M306092200 PubMed 14660642