pAG424-ccdB
(Plasmid
#70124)
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Purpose(Empty Backbone) Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS424-GPD-ccdB
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Backbone manufacturerAddgene Yeast Gateway collection (Lindquist / Gitler)
- Backbone size (bp) 7574
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Modifications to backboneRemoved GPD promoter by SacI/SpeI digestion, gel purification of large product and religation.
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Vector typeYeast Expression
- Promoter None
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Selectable markersTRP1 ; Chloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG424-ccdB was a gift from Jay Hesselberth (Addgene plasmid # 70124 ; http://n2t.net/addgene:70124 ; RRID:Addgene_70124) -
For your References section:
Genetic bypass of essential RNA repair enzymes in budding yeast. Cherry PD, White LK, York K, Hesselberth JR. RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 Dec 6. 10.1261/rna.061788.117 PubMed 29212664
