Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6)
(Plasmid #70232)


Item Catalog # Description Quantity Price (USD)
Plasmid 70232 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    The 3HA coding sequencing was dropped out of backbone with AscI and PacI and replaced with 3FLAG-MNase.
  • Vector type
    Yeast genomic targeting
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    3xFLAG tag and aa 83-231 of Mnase
  • Alt name
    ChEC-tagging vector
  • Alt name
  • Insert Size (bp)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's QC results indicate the selection marker may be a different version of HIS than found in the full sequence, but the depositor notes this does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6) was a gift from Steven Henikoff (Addgene plasmid # 70232 ; ; RRID:Addgene_70232)
  • For your References section:

    ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo. Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S. Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733. 10.1038/ncomms9733 PubMed 26490019