pRL1075
(Plasmid
#70688)
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Purpose(Empty Backbone) Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonesee comments
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Selectable markersErythromycin in cyanobacteria
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites
sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304).
The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL1075 was a gift from Peter Wolk (Addgene plasmid # 70688 ; http://n2t.net/addgene:70688 ; RRID:Addgene_70688) -
For your References section:
Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Black TA, Cai Y, Wolk CP. Mol Microbiol. 1993 Jul;9(1):77-84. PubMed 8412673
Map uploaded by the depositor.
