pGM55
(Plasmid #71249)

• Purpose: luciferase reporter with hGM-CSF -55 to +28 minimal promoter
• Depositing Lab: Peter Cockerill
• Publication: Bert et al Plasmid. 2000 Sep;44(2):173-82.

Backbone

• Vector backbone: pXPG
• Backbone manufacturer: Cockerill lab, Addgene plasmid 71248
• Total vector size (bp): 6188
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GM-CSF minimal promoter (-55 to +28)
• Alt name: colony stimulating factor 2 (granulocyte-macrophage)
• Species: H. sapiens (human)
• Entrez Gene: CSF2 (a.k.a. CSF, GMCSF)
• Promoter: GM-CSF minimal
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

JM109 are the cells used by the author for pXPG and its derivatives.