pETa(+) HpCsd3 (42-403)
(Plasmid #71285)

• Purpose: Expresses Csd3 from Helicobacter pylori
• Depositing Lab: SeWon Suh
• Publication: An et al Acta Crystallogr D Biol Crystallogr. 2015 Mar;71(Pt 3):675-86. doi: 10.1107/S1399004715000152. Epub 2015 Feb 26.

Backbone

• Vector backbone: pET21a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6529
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: To produce protein, transform into Rosetta2(DE3)pLysS cells.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HP0506
• Insert Size (bp): 1086
• Entrez Gene: HP0506 (a.k.a. HP0506)
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry