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pBABEneo-HRASV12
(Plasmid #71304)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71304 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-neo
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-RAS-V12
  • Alt name
    HRAS
  • Alt name
    HRASV12
  • Species
    H. sapiens (human)
  • Mutation
    G12V mutation
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The insert within this plasmid contains a E143K mutation compared to NP_005334.1. The depositor states that this mutation should not affect plasmid function.

The HRAS coding sequence may have been codon optimized and differs from the NCBI reference sequences for this gene. Primer design and applications involving the use of the HRAS coding sequence should be based off of the Addgene NGS Result for accuracy

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEneo-HRASV12 was a gift from Jayanta Debnath (Addgene plasmid # 71304 ; http://n2t.net/addgene:71304 ; RRID:Addgene_71304)
  • For your References section:

    Autophagy facilitates glycolysis during Ras-mediated oncogenic transformation. Lock R, Roy S, Kenific CM, Su JS, Salas E, Ronen SM, Debnath J. Mol Biol Cell. 2011 Jan 15;22(2):165-78. doi: 10.1091/mbc.E10-06-0500. Epub 2010 Nov 30. 10.1091/mbc.E10-06-0500 PubMed 21119005