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pBABEpuro-HA-p62
(Plasmid #71305)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71305 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Addgene plasmid # 1764
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sequestosome-1
  • Alt name
    p62
  • Alt name
    SQSTM1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SQSTM1 (a.k.a. A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human p62 cDNA was a kind gift from Dr. T. Johansen.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a T375A mutation within the Sequestosome-1 translation compared to NP_003891.1. The depositor states that this mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro-HA-p62 was a gift from Jayanta Debnath (Addgene plasmid # 71305 ; http://n2t.net/addgene:71305 ; RRID:Addgene_71305)
  • For your References section:

    Autophagy restricts proliferation driven by oncogenic phosphatidylinositol 3-kinase in three-dimensional culture. Chen N, Eritja N, Lock R, Debnath J. Oncogene. 2013 May 16;32(20):2543-54. doi: 10.1038/onc.2012.277. Epub 2012 Jul 9. 10.1038/onc.2012.277 PubMed 22777351