Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET151-hUCH37 (ISF1)(M148D/F149D)
(Plasmid #71388)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71388 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET151-D-TOPO
  • Backbone manufacturer
    Invotrogen
  • Backbone size w/o insert (bp) 5800
  • Total vector size (bp) 6800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hUCH37 (ISF1) M148D/F149D
  • Alt name
    UCHL5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    989
  • Mutation
    changed methionine 148 to aspartate, phenylalanine 149 to aspartate
  • GenBank ID
    AAD31528.1 AAD31528.1
  • Entrez Gene
    UCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS-TEV sequence (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

M148D/F149D mutations were incorporated via Quickchange mutagenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-hUCH37 (ISF1)(M148D/F149D) was a gift from Chris Hill (Addgene plasmid # 71388 ; http://n2t.net/addgene:71388 ; RRID:Addgene_71388)
  • For your References section:

    Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872