pET151-hUCH37 (ISF1)(M148D/F149D)
(Plasmid
#71388)
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PurposeExpression of hUCH37 (M148D/F149D) in E.coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET151-D-TOPO
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Backbone manufacturerInvotrogen
- Backbone size w/o insert (bp) 5800
- Total vector size (bp) 6800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehUCH37 (ISF1) M148D/F149D
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Alt nameUCHL5
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SpeciesH. sapiens (human)
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Insert Size (bp)989
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Mutationchanged methionine 148 to aspartate, phenylalanine 149 to aspartate
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GenBank IDAAD31528.1 AAD31528.1
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Entrez GeneUCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
- Promoter T7
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Tag
/ Fusion Protein
- HIS-TEV sequence (N terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
M148D/F149D mutations were incorporated via Quickchange mutagenesis.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET151-hUCH37 (ISF1)(M148D/F149D) was a gift from Chris Hill (Addgene plasmid # 71388 ; http://n2t.net/addgene:71388 ; RRID:Addgene_71388) -
For your References section:
Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872