PurposeEncodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71466||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5289
- Total vector size (bp) 7059
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Gene/Insert nameSUMO1/sentrin specific peptidase 2
SpeciesH. sapiens (human)
Insert Size (bp)1770
MutationGene optimised for E. coli expression (Genscript)
Entrez GeneSENP2 (a.k.a. AXAM2, SMT3IP2)
- Promoter T7
/ Fusion Protein
- Hexahistidine (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Insert gene optimisation performed by Genscript
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-HsSENP2 was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 71466 ; http://n2t.net/addgene:71466 ; RRID:Addgene_71466)
For your References section:Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases. Mendes AV, Grou CP, Azevedo JE, Pinto MP. Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 10.1016/j.bbamcr.2015.10.020. 10.1016/j.bbamcr.2015.10.020 PubMed 26522917