pGL4-Gateway-SCP1
(Plasmid #71510)

• Purpose: (Empty Backbone) humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter
• Depositing Lab: Alexander Stark
• Publication: Arnold et al Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. Epub 2013 Jan 17.

Backbone

• Vector backbone: pGL4.10
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression ; humanSTARR-seq validation vector
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pGL4-Gateway-SCP1
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).

Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII