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pGL4-Gateway-SCP1
(Plasmid #71510)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71510 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression ; humanSTARR-seq validation vector
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Resource Information

Depositor Comments

Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).

Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4-Gateway-SCP1 was a gift from Alexander Stark (Addgene plasmid # 71510)
  • For your References section:

    Genome-wide quantitative enhancer activity maps identified by STARR-seq. Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A. Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. Epub 2013 Jan 17. 10.1126/science.1232542 PubMed 23328393