pJK289
(Plasmid #71553)

• Purpose: Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)
• Depositing Lab: T. J. Kappock
• Publication: Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4711
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alanine racemase
• Alt name: alr
• Alt name: AZ09_RS09230
• Species: Acetobacter aceti 1023
• Mutation: changes lysine-39 to alanine (please see depositor's comment below)
• GenBank ID: WP_042787947.1 ABC71322.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains 49 additional nucleotides between the NdeI cloning site and the initiator Met codon