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pEGFP-C3_SEPT6
(Plasmid #71583)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71583 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6008
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Septin 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1281
  • Mutation
    V206D
  • Entrez Gene
    SEPT6 (a.k.a. SEP2, SEPT2)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C3_SEPT6 was a gift from Elias Spiliotis (Addgene plasmid # 71583 ; http://n2t.net/addgene:71583 ; RRID:Addgene_71583)
  • For your References section:

    Septin-driven coordination of actin and microtubule remodeling regulates the collateral branching of axons. Hu J, Bai X, Bowen JR, Dolat L, Korobova F, Yu W, Baas PW, Svitkina T, Gallo G, Spiliotis ET. Curr Biol. 2012 Jun 19;22(12):1109-15. doi: 10.1016/j.cub.2012.04.019. Epub 2012 May 17. 10.1016/j.cub.2012.04.019 PubMed 22608511