pcDNA-HSF1Δ∼PRD
(Plasmid #71725)

• Purpose: plasmid expressing mic-his tag phosphorylation-deficient hHSF1
• Depositing Lab: Lea Sistonen
• Publication: Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11.

Backbone

• Vector backbone: pcDNA 3.1/Myc-His (-) A
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phosphorylation-deficient HSF1
• Alt name: HSF1Δ∼PRD
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: 15 phosphorylation sites in RD mutated to Alanine
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Tag / Fusion Protein:
  • mac 6xHIS (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry