pJK573
(Plasmid #72456)

• Purpose: Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
• Depositing Lab: T. J. Kappock
• Publication: Additional purEK associated constructs (unpublished)

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 3616
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide mutase
• Alt name: purE
• Alt name: YPO3076
• Species: Yersinia pestis strain CO92
• Mutation: synthetic gene with ~57 silent substitutions
• GenBank ID: YP_002347996.1
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (N terminal on insert)
  • TEV cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Database annotation "phosphoribosylaminoimidazole carboxylase catalyic subuni" is incorrect.