pBabe HA-∆Raf1(S642A):ER* puro
(Plasmid #72572)

• Purpose: Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.
• Depositing Lab: Kevin Janes
• Publication: Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Backbone

• Vector backbone: pBabe ER* puro
• Backbone manufacturer: Martin McMahon
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Raf1
• Alt name: CRAF
• Alt name: Raf-1
• Alt name: c-Raf
• Species: H. sapiens (human)
• Insert Size (bp): 2037
• Mutation: amino acids 306-648, serine 642 mutated to alanine
• GenBank ID: NM_002880
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • ER* (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Deborah Morrison

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry