hGDF8 donor CFP-hDF
(Plasmid #72591)

• Purpose: Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.
• Depositing Lab: Renzhi Han
• Publication: Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 10.1038/mtna.2013.39.

Backbone

• Vector backbone: AAVS1 SA-2A-puro-pA donor
• Backbone manufacturer: Rudolf Jaenisch (Addgene plasmid # 22075)
• Backbone size w/o insert (bp): 6602
• Total vector size (bp): 14889
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dysferlin
• Species: H. sapiens (human)
• Entrez Gene: DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
• Promoter: CMV
• Tag / Fusion Protein:
  • ECFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: hGH-PA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette. ccdB gene is nonfunctional.