pEGFP-2A-TAL6-MSTN
(Plasmid #72597)

• Purpose: MSTN TALEN with 2A-GFP tagged
• Depositing Lab: Renzhi Han
• Publication: Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 10.1038/mtna.2013.39.

Backbone

• Vector backbone: pTAL6
• Backbone manufacturer: Han lab
• Vector type: Mammalian Expression, TALEN
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TALEN for MSTN
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To streamline the assembly of a functional pair of TALENs into one plasmid, we placed two GoldyTALEN scaffolds linked by a self-cleaving 2A peptide into a final plasmid designated as pTAL6, which has a unique BsaI site instead of BsmBI site within the second GoldyTALEN scaffold.