pKPY115
(Plasmid
#72669)
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PurposeEncodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD33
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Backbone manufacturerBeckwith Lab
- Backbone size w/o insert (bp) 5319
- Total vector size (bp) 8717
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameThr251Gly-EcPheRS
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Alt nameE. coli Phenylalanyl-tRNA Synthetase (Thr251Gly in Alpha Subunit)
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SpeciesE. coli
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Insert Size (bp)3398
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MutationThr251Gly in Alpha Subunit
- Promoter PBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKPY115 was a gift from David Tirrell (Addgene plasmid # 72669 ; http://n2t.net/addgene:72669 ; RRID:Addgene_72669)
