|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72673||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2544
- Total vector size (bp) 5358
Vector typeGateway Entry Clone
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameThr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
SpeciesC. elegans (nematode)
Insert Size (bp)2814
MutationThr412Gly in CePheRS Alpha Subunit
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKPY737 was a gift from David Tirrell (Addgene plasmid # 72673)