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PurposeExpress Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDF-1b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas1, Cas2
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Speciesfrom K12 genome
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Insert Size (bp)1204
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCas1+2 was a gift from Udi Qimron (Addgene plasmid # 72676 ; http://n2t.net/addgene:72676 ; RRID:Addgene_72676) -
For your References section:
Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli. Yosef I, Goren MG, Qimron U. Nucleic Acids Res. 2012 Jul;40(12):5569-76. doi: 10.1093/nar/gks216. Epub 2012 Mar 8. 10.1093/nar/gks216 PubMed 22402487
