PatoG-OG241
(Plasmid #72839)

• Purpose: Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
• Depositing Lab: Chris Barnes
• Publication: Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 10.1002/bit.27897. Epub 2021 Aug 9.

Backbone

• Vector backbone: OG241
• Backbone manufacturer: Oxford Genetics
• Total vector size (bp): 4719
• Modifications to backbone: Inserted PatoG promoter upstream daGFP
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PatoG
• Species: E. coli DH5A
• Insert Size (bp): 576
• Promoter: PatoG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: TAAGCAACTAGTGCCGTGCATTGATGTATAAACT
• 3′ sequencing primer: TGCTTAGGTACCGTGGCGTCTTGTAATGTCAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Capillary sequence files used the primers CATCTTCGATGGATAGCGATT and TGTACGGGATCTCCTTCTCG to verify MCS region including the promoter and the start codon of daGFP.

Please visit https://www.biorxiv.org/content/early/2016/01/07/035972 for bioRxiv preprint.