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pMHG-W57* reporter
(Plasmid #72850)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72850 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMONOhygro-gfp (pMHG)
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 4728
  • Modifications to backbone
    Site-directed mutagenesis of GFP tryptophan 57 codon to a stop codon (W57⁄) (G171A, mRNA). An opal stop codon (TGA) was introduced using the following primers (forward, G CTG CCA GTG CCT TGA CCA ACT CTG GTG AC; reverse, GTC ACC AGA GTT GGT CAA AGG CAC TGG CAG C).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMHG-W57* reporter was a gift from Daniel Liebler (Addgene plasmid # 72850 ; http://n2t.net/addgene:72850 ; RRID:Addgene_72850)
  • For your References section:

    A Reporter System for Translational Readthrough of Stop Codons in Human Cells. Halvey PJ, Liebler DC, Slebos RJ. FEBS Open Bio. 2012 Dec;2:56-59. Epub 2012 Apr 17. 10.1016/j.fob.2012.04.004 PubMed 22563532