PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72891||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepWPRE/C1 (modified pEGFP-C1)
Backbone manufacturerModified from Clontech
- Backbone size w/o insert (bp) 4581
- Total vector size (bp) 7470
Modifications to backboneThe woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was inserted into the BamHI site of pEGFP-C1, and EGFP was removed.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human); Avena sativa (oat)
Insert Size (bp)2889
- Promoter CMV
/ Fusion Protein
- IRES-GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hBACCS2-IRES-GFP was a gift from Takao Nakata (Addgene plasmid # 72891 ; http://n2t.net/addgene:72891 ; RRID:Addgene_72891)
For your References section:Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514