pLV-NT-3/D15A
(Plasmid #73035)

• Purpose: Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells
• Depositing Lab: Pantelis Tsoulfas
• Publication: Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.

Backbone

• Vector backbone: pRRLsinPPT.CMV.MCS.Wpre
• Backbone manufacturer: Naldini
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 7600
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Grow on low salt, 5gm, of LB
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NT-3/D15A
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Mutation: Mutated Aspartic acid in position 15 of the mature NT-3 to alanine. With this mutation NT-3 can bind also TrkB.
• Entrez Gene: NTF3 (a.k.a. HDNF, NGF-2, NGF2, NT-3, NT3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI and BamHI (not destroyed)
• 3′ cloning site: none (destroyed during cloning)
• 5′ sequencing primer: cmv_frw_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry