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pLV-NT-3/D15A
(Plasmid #73035)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73035 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRLsinPPT.CMV.MCS.Wpre
  • Backbone manufacturer
    Naldini
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 7600
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow on low salt, 5gm, of LB
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NT-3/D15A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    Mutated Aspartic acid in position 15 of the mature NT-3 to alanine. With this mutation NT-3 can bind also TrkB.
  • Entrez Gene
    NTF3 (a.k.a. HDNF, NGF-2, NGF2, NT-3, NT3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI and BamHI (not destroyed)
  • 3′ cloning site none (destroyed during cloning)
  • 5′ sequencing primer cmv_frw_primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-NT-3/D15A was a gift from Pantelis Tsoulfas (Addgene plasmid # 73035 ; http://n2t.net/addgene:73035 ; RRID:Addgene_73035)
  • For your References section:

    A multifunctional neurotrophin with reduced affinity to p75NTR enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Enomoto M, Bunge MB, Tsoulfas P. Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20. 10.1016/j.expneurol.2013.06.013 PubMed 23792206