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pLPC myc-mRAP1
(Plasmid #73136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73136 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 73136-D50 50 μg of DNA in Tris buffer $495
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DNA 73136-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pLPC-myc
  • Backbone manufacturer
    de Lange lab (Addgene plasmid #12540)
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rap1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Entrez Gene
    Terf2ip (a.k.a. Rap1)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pLPC-5 (5'-TAGGCGTGTACGGTGGGA-3')
  • 3′ sequencing primer pLPC-3 (5'-CTTGCCAAACCTACAGGT-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional reference: Cell Rep. 2013 Jun 27;3(6):1847-56.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC myc-mRAP1 was a gift from Titia de Lange & Agnel Sfeir (Addgene plasmid # 73136 ; http://n2t.net/addgene:73136 ; RRID:Addgene_73136)

  • For your References section:

    Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Sfeir A, Kabir S, van Overbeek M, Celli GB, de Lange T. Science. 2010 Mar 26;327(5973):1657-61. doi: 10.1126/science.1185100. 10.1126/science.1185100 PubMed 20339076
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