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pCAS1yl
(Plasmid #73226)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73226 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMCSCen1
  • Vector type
    Bacterial Expression, CRISPR ; in Y. lipolytica
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes MGAS5005
  • Promoter unknown

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F20
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Species
    Synthetic

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Hal S.Alper , The University of Texas at Austin,Austin,TX,USA
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS1yl was a gift from Sheng Yang (Addgene plasmid # 73226 ; http://n2t.net/addgene:73226 ; RRID:Addgene_73226)
  • For your References section:

    Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S. J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27. 10.1007/s10295-016-1789-8 [pii] PubMed 27349768