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KDM4D
(Plasmid #73247)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73247 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    SGC, Addgene Plasmid #26103
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    KDM4D
  • Alt name
    JMJD2DA-c105
  • Alt name
    gi|39653317
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1120
  • Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
  • Entrez Gene
    KDM4D (a.k.a. JMJD2D)
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Use BL21(DE3)-R3-pRARE2 for protein expression. See also 4D6Q: http://www.thesgc.org/structures/4D6Q 4D6R: http://www.thesgc.org/structures/4D6R 4D6S: http://www.thesgc.org/structures/4D6S

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KDM4D was a gift from Nicola Burgess-Brown (Addgene plasmid # 73247 ; http://n2t.net/addgene:73247 ; RRID:Addgene_73247)