pJK294
(Plasmid #73373)

• Purpose: Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
• Depositing Lab: T. J. Kappock
• Publication: Additional purEK associated constructs (unpublished)

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 4176
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide mutase
• Alt name: purE
• Alt name: AZ09_02690
• Species: Acetobacter aceti 1023
• Mutation: changed histidine-59 to aspartate and histidine-89 to aspartate, additional N-terminal methionine
• GenBank ID: AAZ04483.1 KDE21029.1
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains an additional silent mutation in Ser57 that adds a nonunique PstI site.