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RabV CVS-N2c(deltaG)-hChR2-YFP
(Plasmid #73465)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 73465 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 73465-D50 50 μg of DNA in Tris buffer $495
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DNA 73465-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Rabies CVS N2c(deltaG)
  • Backbone manufacturer
    Matthias J. Schnell, Thomas Jefferson University
  • Backbone size w/o insert (bp) 14070
  • Total vector size (bp) 15792
  • Vector type
    Neurotropic virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can also be grown in Stbl2 or Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hChR2-YFP
  • Insert Size (bp)
    1731

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RabV CVS-N2c(deltaG)-hChR2-YFP was a gift from Thomas Jessell (Addgene plasmid # 73465 ; http://n2t.net/addgene:73465 ; RRID:Addgene_73465)

  • For your References section:

    Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A. Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. 10.1016/j.neuron.2016.01.004 PubMed 26804990
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