pEGFP-ninein coiled-coil 1
(Plasmid
#73521)
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PurposeEncodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus
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Depositing Lab
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Depositing OrganizationInstitut Curie
Located in France -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 73521-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 73521-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameninein coiled-coil 1
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SpeciesM. musculus (mouse)
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Entrez GeneNin (a.k.a. 3110068G20Rik, AI385615, AU024711, mKIAA1565)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP 3' Fw
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by Nathalie DELGEHYR
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 73521-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 73521-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-ninein coiled-coil 1 was a gift from Michel Bornens (Addgene plasmid # 73521 ; http://n2t.net/addgene:73521 ; RRID:Addgene_73521) -
For your References section:
Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. Delgehyr N, Sillibourne J, Bornens M. J Cell Sci. 2005 Apr 15;118(Pt 8):1565-75. Epub 2005 Mar 22. 10.1242/jcs.02302 PubMed 15784680