Plekhm1-N terminal pFastHFTb
(Plasmid
#73828)
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PurposeExpress human Plekhm1 N-part in insect cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac-HFb
- Backbone size w/o insert (bp) 4857
- Total vector size (bp) 6347
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePlekhm1
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SpeciesH. sapiens (human)
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Insert Size (bp)1490
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Mutationcloned N- terminal part of human Plekhm1 (aa 1- 497)
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Entrez GenePLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer pFastForward
- 3′ sequencing primer pFast reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Plekhm1-N terminal pFastHFTb was a gift from Paul Odgren (Addgene plasmid # 73828 ; http://n2t.net/addgene:73828 ; RRID:Addgene_73828)