FU - dio YFP - W
(Plasmid #73858)

• Purpose: For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.
• Depositing Lab: Elly Nedivi
• Publication: Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010.

Backbone

• Vector backbone: pFUGW
• Backbone size w/o insert (bp): 9421
• Total vector size (bp): 10138
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eYFP
• Alt name: enhanced yellow fluorescent protein
• Species: Synthetic
• Insert Size (bp): 717

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Jerry Chen
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this backbone is prone to recombination. Addgene recommends that, upon receipt of this plasmid, users please streak onto an LB plate and screen single colonies by restriction digest before subsequent growth, storage, and experimentation.

This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).