pUAST attB BmOr2 (Y464A)
(Plasmid
#73927)
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PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA with 464 amino acid change of Tyrosine to Alanine
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUAST attB
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBmOr2 (Y464A)
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SpeciesBombyx mori
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Insert Size (bp)1000
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Mutationchanged 464 Tyrosine to Alanine
- Promoter hsp70
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
- 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST attB BmOr2 (Y464A) was a gift from Leslie Vosshall (Addgene plasmid # 73927 ; http://n2t.net/addgene:73927 ; RRID:Addgene_73927) -
For your References section:
Amino acid residues contributing to function of the heteromeric insect olfactory receptor complex. Nakagawa T, Pellegrino M, Sato K, Vosshall LB, Touhara K. PLoS One. 2012;7(3):e32372. doi: 10.1371/journal.pone.0032372. Epub 2012 Mar 5. 10.1371/journal.pone.0032372 PubMed 22403649
