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pBluescriptII[attB/Ins1]
(Plasmid #74100)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74100 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3618
  • Modifications to backbone
    A small piece of pSPORT1 containing an attB element and a dual chicken beta-globin Hyper Sensitive Site 4 (HS4) insulator core elements was cloned into the ClaI/SpeI sites of pBluescript II SK (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Phi C31 attb element
  • Insert Size (bp)
    27

Gene/Insert 2

  • Gene/Insert name
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    510

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescriptII[attB/Ins1] was a gift from Ben Szaro (Addgene plasmid # 74100 ; http://n2t.net/addgene:74100 ; RRID:Addgene_74100)
  • For your References section:

    A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles. Wang C, Szaro BG. Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20. 10.1016/j.ydbio.2014.11.010 PubMed 25448690