pJKW 0633
(Plasmid
#74122)
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PurposePhotinus pyralis sulfotransferase ST2 - pHis8-4
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHis8-4
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Backbone manufacturerProduced in Jing-Ke Weng lab
- Backbone size w/o insert (bp) 5350
- Total vector size (bp) 6456
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePhotinus pyralis sulfotransferase 2
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Alt nameST2
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Insert Size (bp)876
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GenBank IDKX097999
- Promoter T7
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Tag
/ Fusion Protein
- 8x-His, TEV protease site (ENLYFQ) (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses robustly in E.coli. Minimal activity for desulfonation of p-nitrophenolsulfate with PAP
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJKW 0633 was a gift from Jing-Ke Weng (Addgene plasmid # 74122 ; http://n2t.net/addgene:74122 ; RRID:Addgene_74122) -
For your References section:
Sulfoluciferin is biosynthesized by a specialized luciferin sulfotransferase in fireflies. Fallon TR, Li FS, Vicent MA, Weng JK. Biochemistry. 2016 May 26. 10.1021/acs.biochem.6b00402 PubMed 27227579
