|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74122||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerProduced in Jing-Ke Weng lab
- Backbone size w/o insert (bp) 5350
- Total vector size (bp) 6456
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namePhotinus pyralis sulfotransferase 2
Insert Size (bp)876
- Promoter T7
/ Fusion Protein
- 8x-His, TEV protease site (ENLYFQ) (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
Expresses robustly in E.coli. Minimal activity for desulfonation of p-nitrophenolsulfate with PAP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJKW 0633 was a gift from Jing-Ke Weng (Addgene plasmid # 74122)
For your References section:Sulfoluciferin is biosynthesized by a specialized luciferin sulfotransferase in fireflies. Fallon TR, Li FS, Vicent MA, Weng JK. Biochemistry. 2016 May 26. 10.1021/acs.biochem.6b00402 PubMed 27227579
Generated by Addgene from full sequence supplied by depositor.