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pCMV-GCaMP3bright
(Plasmid #74423)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74423 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3945
  • Total vector size (bp) 5308
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP3bright
  • Alt name
    GCaMP3 EF-4
  • Species
    R. norvegicus (rat); A. victoria (jellyfish)
  • Insert Size (bp)
    1363
  • Mutation
    D392A
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GCaMP3bright was a gift from Katalin Torok (Addgene plasmid # 74423 ; http://n2t.net/addgene:74423 ; RRID:Addgene_74423)
  • For your References section:

    Fast-Response Calmodulin-Based Fluorescent Indicators Reveal Rapid Intracellular Calcium Dynamics. Helassa N, Zhang XH, Conte I, Scaringi J, Esposito E, Bradley J, Carter T, Ogden D, Morad M, Torok K. Sci Rep. 2015 Nov 3;5:15978. doi: 10.1038/srep15978. 10.1038/srep15978 PubMed 26527405