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pHR-mCherry-p2A-LIC1 C-terminal half
(Plasmid #74603)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74603 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR-mCherry-p2A
  • Backbone size w/o insert (bp) 9757
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    full length human dynein light intermediate chain 1 amino acids 389-523
  • Alt name
    LIC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    438
  • Mutation
    amino acids 389-523 of LIC1
  • Entrez Gene
    DYNC1LI1 (a.k.a. DLC-A, DNCLI1, LIC1)
  • Promoter SFFV
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-mCherry-p2A-LIC1 C-terminal half was a gift from Ron Vale (Addgene plasmid # 74603 ; http://n2t.net/addgene:74603 ; RRID:Addgene_74603)
  • For your References section:

    A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. Schroeder CM, Ostrem JM, Hertz NT, Vale RD. Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351. 10.7554/eLife.03351 PubMed 25272277