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pET28a-Hook3 I136A, I139A, M142A
(Plasmid #74617)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74617 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 6198
  • Total vector size (bp) 6915
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3) for protein production
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human Hook3 amino acids 1-239, I136A, I139A, M142A
  • Alt name
    HOOK3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    717
  • Mutation
    D102A, E108A
  • GenBank ID
    NM_032410
  • Entrez Gene
    HOOK3 (a.k.a. HK3)
  • Promoter T7
  • Tags / Fusion Proteins
    • superfolder GFP (N terminal on insert)
    • Strep II (N terminal on insert)
    • 6x His (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Hook3 I136A, I139A, M142A was a gift from Ron Vale (Addgene plasmid # 74617 ; http://n2t.net/addgene:74617 ; RRID:Addgene_74617)
  • For your References section:

    Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3. Schroeder CM, Vale RD. J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002. 10.1083/jcb.201604002 PubMed 27482052