|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74666||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Alt nameBRD1bromodomain containing protein 1
SpeciesH. sapiens (human)
Insert Size (bp)478
Entrez GeneBRD1 (a.k.a. BRL, BRPF1, BRPF2)
- Promoter T7
/ Fusion Protein
- His-6-TEV (N terminal on backbone)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|11321642
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BRD1A was a gift from Nicola Burgess-Brown (Addgene plasmid # 74666 ; http://n2t.net/addgene:74666 ; RRID:Addgene_74666)