pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
(Plasmid
#74919)
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PurposeMammalian expression of mKlf2 and mNanog-mCherry
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74919 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 74919-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 74919-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPB-CAG
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameKlf2
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SpeciesM. musculus (mouse)
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Entrez GeneKlf2 (a.k.a. Lklf)
- Promoter CAG
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Tag
/ Fusion Protein
- T2A (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameNanog
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SpeciesM. musculus (mouse)
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Entrez GeneNanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 74919-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 74919-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo was a gift from Jose Silva (Addgene plasmid # 74919 ; http://n2t.net/addgene:74919 ; RRID:Addgene_74919) -
For your References section:
MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. dos Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, Hendrich B, Silva JC. Cell Stem Cell. 2014 Jul 3;15(1):102-10. doi: 10.1016/j.stem.2014.04.019. Epub 2014 May 15. 10.1016/j.stem.2014.04.019 PubMed 24835571