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PurposeClone encoding rabbit ryanodine receptor in mammalian expression vector
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Depositing Labs
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Depositing OrganizationBrigham and Women's Hospital
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 75113-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 75113-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCIneo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5462
- Total vector size (bp) 20594
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRyanodine receptor 1
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Alt nameRYR1
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SpeciesRabbit
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Insert Size (bp)15132
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GenBank IDNM_001101718
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Paul D Allen, UC Davis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 75113-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 75113-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIneo RYR-1 was a gift from Paul Allen & Robert Dirksen (Addgene plasmid # 75113 ; http://n2t.net/addgene:75113 ; RRID:Addgene_75113) -
For your References section:
Two regions of the ryanodine receptor involved in coupling with L-type Ca2+ channels. Nakai J, Sekiguchi N, Rando TA, Allen PD, Beam KG. J Biol Chem. 1998 May 29;273(22):13403-6. PubMed 9593671
