Rat SERCA1a (pMT2)
(Plasmid
#75182)
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PurposeMammalian expression of ATP2A1
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Depositing Lab
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Depositing OrganizationBrigham and Women's Hospital
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 75182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 75182-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 75182-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMT2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP2A1
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Alt nameSERCA1A
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SpeciesR. norvegicus (rat)
- Promoter adenovirus major late promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F
- 3′ sequencing primer pMT2-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 75182-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 75182-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rat SERCA1a (pMT2) was a gift from Jonathan Lytton (Addgene plasmid # 75182 ; http://n2t.net/addgene:75182 ; RRID:Addgene_75182) -
For your References section:
Molecular cloning and quantification of sarcoplasmic reticulum Ca(2+)-ATPase isoforms in rat muscles. Wu KD, Lytton J. Am J Physiol. 1993 Feb;264(2 Pt 1):C333-41. 10.1152/ajpcell.1993.264.2.C333 PubMed 8447366
