PXL
(Plasmid #75349)

• Purpose: PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
• Depositing Lab: Bryan Luikart
• Publication: Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.

Backbone

• Vector backbone: PX330
• Total vector size (bp): 8548
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas9
• gRNA/shRNA sequence: Empty Cassette
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstB1 or Pac1 (not destroyed)
• 3′ cloning site: Pac1 (not destroyed)
• 5′ sequencing primer: LKO1

Resource Information

• Supplemental Documents:
  • PXL
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from PX330