PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78100||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 4387
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Copy numberHigh Copy
Insert Size (bp)1728
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (unknown if destroyed)
- 3′ cloning site SacI (unknown if destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byP. Xu PMID: 22581370. The Chloramphenicol gene was cloned from the pKD3 plasmid (Keio Collection, CGSC #7631). PubMed 10829079
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUCmEos3.2:cat was a gift from Peng Chen (Addgene plasmid # 78100 ; http://n2t.net/addgene:78100 ; RRID:Addgene_78100)
For your References section:Concentration- and chromosome-organization-dependent regulator unbinding from DNA for transcription regulation in living cells. Chen TY, Santiago AG, Jung W, Krzeminski L, Yang F, Martell DJ, Helmann JD, Chen P. Nat Commun. 2015 Jul 6;6:7445. doi: 10.1038/ncomms8445. 10.1038/ncomms8445 PubMed 26145755
Map uploaded by the depositor.