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pUCmEos3.2:cat
(Plasmid #78100)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78100 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4387
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEos3.2-cat
  • Insert Size (bp)
    1728

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    P. Xu PMID: 22581370. The Chloramphenicol gene was cloned from the pKD3 plasmid (Keio Collection, CGSC #7631). PubMed 10829079
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUCmEos3.2:cat was a gift from Peng Chen (Addgene plasmid # 78100 ; http://n2t.net/addgene:78100 ; RRID:Addgene_78100)
  • For your References section:

    Concentration- and chromosome-organization-dependent regulator unbinding from DNA for transcription regulation in living cells. Chen TY, Santiago AG, Jung W, Krzeminski L, Yang F, Martell DJ, Helmann JD, Chen P. Nat Commun. 2015 Jul 6;6:7445. doi: 10.1038/ncomms8445. 10.1038/ncomms8445 PubMed 26145755