pUCmEos3.2:cat
(Plasmid #78100)

• Purpose: Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
• Depositing Lab: Peng Chen
• Publication: Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1038/ncomms8445.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 4387
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mEos3.2-cat
• Insert Size (bp): 1728

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (unknown if destroyed)
• 3′ cloning site: SacI (unknown if destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: P. Xu PMID: 22581370. The Chloramphenicol gene was cloned from the pKD3 plasmid (Keio Collection, CGSC #7631). PubMed 10829079

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry